Hōʻikeʻike Lactoperoxidase

Lactoperoxidase (LPO), ka mea i ʻike ʻia i nā moena salivary a me nā momona mammary, he mea koʻikoʻi o ka pane ʻalike he mea nui i ka mālama pono ʻana i ke olakino maikaʻi. ʻO ka hana nui o ka lactoperoxidase ʻo ka hoʻokaʻawale i nā ʻonika thiocyanate (SCN−) i loaʻa i ka lawena ma ke alo o ka hydrogen peroxide e hopena i nā huahana e hōʻike i ka hana antimicrobial. Ua loaʻa ʻo LPO i loko o ka waiū bovine i loko o nā ʻoihana olakino, ʻai, a me nā mea hoʻonaninani ma muli o ka hana like a me ka hoʻokūkū o ka enzyme o ke kanaka.

Hoʻonui ʻia nā huahana ʻekikika oral modern me ka lactoperoxidase hāpana e hāʻawi i kahi koho maikaʻi loa no ka niho fluoride maʻamau. No ka nui o nā noi ākea o ka lactoperoxidase hoʻohui, ua hoʻonui nui ʻia kona noi i nā makahiki, a e ulu pū ana nō.
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He aha ka Lactoperoxidase?

ʻO Lactoperoxidase wale nō ka enzyme peroxidase i hana ʻia mai ka mucosal, mammary, a me nā salivary kūloko, e hana ana he mālamuku anti-bacteria. I nā kanaka, ua hoʻopili ʻia ka waihona lactoperoxidase e ka LPO gene. Loaʻa kēia enzyme i nā mammalians, e komo pū me nā kānaka, nā ʻiole, bovine, kāmela, poʻokela, bipi, kao, Ilama, a me nā hipa.

Lactoperoxidase Pūnaewele:

LPO he mea lawelawe maikaʻi e antimicrobial. Ke hoʻohana nei ka Lactoperoxidase, ma muli o kēia lula. Loaʻa ka noi Lactoperoxidase ma ka nui o ka mālama ʻana i nā meaʻai, nā hoʻonā ophthalmic, a me nā kumu hana hoʻonā. Eia kekahi, hoʻohana ka lactoperoxidase pauku i ka lāʻau lapaʻau a me ka mālama niho. Eia kekahi, ʻo LPO kahi hana anti-viral a me ka anti-tumor. Ua kūkākūkā ʻia ma lalo nei he mea hoʻohana hou ka lactoperoxidase:

i. ʻO ka maʻi kanulau

He maʻi kanesa Lactoperoxidase pili me ka hiki ke hoʻokele waiwai me ka hiki iā ia ke pale i ka estradiol. Ke alakaʻi nei kēia oxidative i ke kaumaha oxidative i loko o nā wahi maʻi kanesa o nā umauma. Lactoperoxidase hana ma laila ke kumu i kahi kaila o ka hoʻoheheʻe i alakaʻi i ka hoʻohana ʻana i ka oxygen a hōʻiliʻili ʻana o ka hydrogen peroxide intracellular. Ma muli o kēia mau hopena, hoʻomaʻemaʻe maikaʻi ʻo LPO i nā ʻōpū kinipiko i ka vitro. Eia nō hoʻi, ua hoʻohana ʻia nā macrophages i hōʻike ʻia i ka LPO e luku ai i nā maʻi kanesa, pepehi lākou iā lākou.

ii. Hoʻopilikia i nā hopena anti-bacteria

ʻO ka pūnao LPO ka hana ma ke ʻano he kūlohelohe o ka ʻoihana pale pale ole o ka maʻi o ka mammals a hoʻomaʻamaʻa aku ia i ka hopena o ka hoʻōla ʻana o ka siʻi thiocyanate i loko o ka antibacterial hypothiocyanate. Hiki i ka LPO ke kāohi i ka ulu o kahi pālahalaha o nā microorganisms ma o kahi hoonui enzymatic e pili ana i nā ʻolika thiocyanate a me hydrogen peroxide e like me nā cofactors. Hoʻololi ʻia ka hana antimicrobial o LPO i ka hoʻokumu ʻana o nā huawai hypothiocyanite ma o ka hoʻōla ʻana o nā huina. Hiki i nā huapalani Hypothiocyanite ke hana me nā membrane bacterial. Hoʻolauna lākou i kahi hana o ka hana metabolic enzim. Pepehi ʻo Lactoperoxidase i nā pale o Gram-negative bacteria a pale aku i ka ulu a me ka hoʻomohala ʻana i nā huakaʻi maikaʻi-Gram.

iii. Lactoperoxidase i ka hoʻonani

ʻO ka hui pūʻana o ka pauma lactoperoxidase, glucose, thiocyanate, iodide,

a me ka hoʻōho glucose, a ʻike ʻia i ke ola ʻia i ka mālama ʻana i nā mea hoʻonaninani.Lactoperoxidas-02

iv. Lactoperoxidase i ka waiū Mālama

ʻO ka hiki i ka lactoperoxidase i ka mālama ʻana i ka maikaʻi o ka waiū waiʻai no ka manawa i hāʻawi ʻia i kahi manawa i hoʻokau ʻia ma nā kīhāpai a me nā hoʻokolohua hoʻokolohua i hana ʻia ma nā wahi kikoʻāina. Hiki ke hoʻohana ʻia ka Lactoperoxidase preservative e mālama i ka waiū wai i loaʻa mai nā ʻano ʻano like ʻole. Pehea ka hopena o ke ala ma nā kumu he nui. Hoʻokomo kēia mau mea i ka waiū wai i ka wā o ka mālama ʻana, ʻano ʻano o ka hakahaka o ka microbiological, a me ka nui o ka waiū.

Hoʻokomo ʻo Lactoperoxidase i ka hopena bacteriostatic ma ka waiū waiū momi. Hōʻike nā ʻikepili a me ka ʻike mai ka hana maoli e hiki ke hoʻohana ʻia ka lactoperoxidase ma mua o nā palena palena o ka (15-30 mau degere Celsius) i ʻōlelo ʻia ma nā kulekele codex o 1991. Ma ka palena haʻahaʻa o ke ana, ua hōʻike ʻia nā haʻawina like i ka hoʻoweliweli ʻana o ka lactoperoxidase hiki ke hoʻolōʻihi i ka ulu o nā waiū waiū psychrotrophic a no laila e hōʻaila i ka hoʻokaʻawale i ka waiū no nā lā hou e hoʻohālikelike i ka pani. He mea koʻikoʻi ia e hoʻomaopopo i ka kumu o ka hoʻohana ʻana i ka lactoperoxidase, ʻaʻole ia e hana i ka waiū palekana no ka hoʻohana ʻana akā e mālama i kāna ʻano kumu.

ʻO ka hoʻomaʻamaʻa ʻana i ka hoʻomaʻemaʻe maikaʻi i ka hana ʻana o ka waiū he mea nui ia no ka ola lactoperoxidase a hiki i ka ʻano waiū microbiological. Hiki ke loaʻa ke palekana a me ka hou o ka waiū ma o ka hui pū ʻana o ka mālama wela i ka waiū a me nā hana maʻemaʻe maikaʻi e kūʻokoʻa ana me ka hoʻohana ʻana i ka lactoperoxidase.

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v. Nā hana'ē aʻe

Ua hōʻike ʻia nā haʻawina ʻo ka hiki ke loaʻa nā hopena antiviral, hiki iā Lactoperoxidase ke pale aku i nā holoholona holoholona mai nā ʻano like ʻole o nā pōʻino a me ka peroxidation, a he mea nui hoʻi ia o ka ʻōnaehana pale ma nā microorganism pathogen i loko o ka digestive system o nā pēpē hou.

ʻŌnaehana Lactoperoxidase

He aha ka ʻōnaehana Lactoperoxidase?

ʻO ka ʻōnaehana Lactoperoxidase (LPS) i hoʻokumu ʻia i ʻekolu mau māhele, kahi o ka lactoperoxidase, hydrogen peroxide, a me thiocyanate (SCN¯). ʻO ka ʻōnaehana Lactoperoxidase he hana anti-bacterial wale nō i ka wā e hana pū ai kēia mau mea ʻekolu. Ma ka hoʻohana maoli ʻana, inā lawa ka ʻike o kekahi mau ʻāpana i loko o ka ʻōnaehana, pono e hoʻohui ʻia ia e hōʻoia i ka hopena anti-bacteria, ka mea i ʻike ʻia o ka hoʻālaʻi LPS. I waena o lākou, ʻaʻole pono ka emiʻana o ka lactoperoxidase ma mua o 0.02 U / mL.

ʻO ka ʻike lactoperoxidase kūlohelohe i ka waiū bovine he 1.4 U / mL, nāna e hālāwai i kēia koi. Loaʻa ākea ka SCN ʻ in i nā mea huna a me nā ʻili o nā holoholona. I loko o ka waiū, ʻo ka hoʻoili o ka thiocyanate e like me ka liʻiliʻi o 3-5 μg / mL. He palena palena kēia no ka hana o ka lawelawe ʻo Lactoperoxidase. Ua manaʻo ʻia e pono thiocyanate i mea e hoʻoulu ai i ka ʻōnaehana lactoperoxidase ma ka 15 μg / mL a i ʻole. ʻO ia ke kumu e pono ai mākou e hoʻohui i kēia exogenous thiocyanate e hoʻolaʻa i ka ʻōnaehana lactoperoxidase. ʻO ka ʻike hydrogen peroxide i ka waiū, ka mea i hoʻopili ʻia, ʻo ia wale nō ʻo 1-2 μg / Ml, a me ka hoʻouluulu ʻana o LPS e pono ka 8-10 g / mL o ka hydrogen peroxide. ʻO ia ke kumu e hoʻolako ʻia ai nā hydrogen peroxide.

ʻO ka ʻōnaehana lactoperoxidase kahi koʻikoʻi i ka hana kūlohelohe, hiki iā ia ke pepehi i nā pōʻino i ka waiū a me nā ʻōlelo huna mucosal a loaʻa paha nā noi therapeutic.

I nā huahana meaʻai a me nā olakino olakino, hoʻohui a hoʻohui ʻia paha i ka ʻōnaehana lactoperoxidase i kekahi mau manawa no ka mālama ʻana i nā huakini.

Pehea ia hana?

ʻO ka LPS e hoʻokumu i ka hoʻohui o ka hoʻohui anti-bacteria mai ka SCN¯catalysed e ka LPO i ka hāʻuleʻana o ka hydrogen peroxide. ʻO ka lactoperoxidase i ʻōlelo ʻia hana antimicrobial ua ʻike maoli ʻia i loko o nā nui o ke ʻano leila e like me ka gastric wai, nā waimaka, a me nā lawena wai. ʻO nā mea waiwai ʻelua no ka ʻōnaehana antimicrobial, ʻo ia ka hydrogen peroxide a me ka thiocyanate, aia lākou i ka waiū i nā kikowaena like ʻole, e pili ana i nā ʻano holoholona a me ka hānai i hāʻawi ʻia.

I loko o ka waiū hou, nāwaliwali ka hana antimicrobial a noho like no ka 2 mau hola mai ka waiū wale nō e loaʻa nā pae suboptimal o ka hydrogen peroxide a me ka thiocyanate ion. Hoʻohui ʻia ʻo Thiocyanate kahi i hoʻopaʻa ʻia i loko o ka hopena 2 electron e hāʻawi ana i ka hypothiocyanite

E hana ana nā Thiocyanate ma o kahi cofactor no ka ʻōnaehana Lactoperoxidase. A ʻo ka hopena, ʻo ka nui o ka oxhidized sulfhydryls i ka huina he kūʻokoʻa o ka ion thiocyanate i ka lōʻihi o ka wā

  1. Loaʻa ka Thiol moiety
  2. ʻAʻole i hoʻopau ʻia ʻo Thiocyanate
  • Nui ka hydrogen peroxide
  1. ʻAʻole i hoʻopili ʻia ʻo Thiocyanate ma kahi ʻano waikawa amino

A ʻo kahi hopena, hoʻomaka ka thiocyanate i ka hopena anti-bacterial o ka ʻōnaehana lactoperoxidase i ka waiū hou. Hoʻopiha ʻia kēia i ke ola o nā waiū hou ma lalo o nā kūlana tropikal no ʻehiku a ʻewalu mau hola.

Hoʻohana / Hoʻohana Lactoperoxidase

i. Nā hana anti-microbial

ʻO ka hana anti-microbial o ka lactoperoxidase system i ʻike ʻia ma ka hana o ka maʻi bactericidal a me ka bacteriostatic o kekahi microorganism i loaʻa i ka waiū maka. Hoʻokumu ka hana bactericidal i ka mea i loaʻa i ka hui thiol ma nā kōnī i loko o nā microbial cell 'membrane ka paʻakai. Hoʻopilikia kēia i ka luku ʻana o ka membrane plasma e alakaʻi i ka leakage o ka polypeptides, pāleona potassium, a me nā ʻaina amino. Hōʻalo ʻia ka paʻakai ʻana a me nā pyrimidines, glucose, a me nā waikawa amino e nā ʻona ke kāohi ʻia. Hoʻomaʻa ʻia ka syntnt o ka DNA, RNA, a me nā protein.

Hōʻike nā maʻiʻokoʻa i ka ʻokoʻa i ka hoʻoweliweli ʻana i ka ʻōnaehana lactoperoxidase. ʻO nā hōʻiliʻili o ka Gram-Negative, e like me Salmonella, Pseudomonas, a me Escherichia coli, ua kālai ʻia a pepehi ʻia. Hoʻokomo ʻia nā bakule acid Lactic a me Streptococcus wale nō. ʻO ka make ʻana o kēia mau bacteria ma ka lactoperoxidase system e hōʻeha nei i kekahi mau meaʻai, ke ʻalū ʻana i nā maʻi mai ka lawe ʻana i nā mea kanu huaʻai, a ke alakaʻi nei kēia i ka hāʻule a i ʻole ka make o nā huakini.

ii. Ka hoʻomaʻemaʻe ʻana i ka paradentosis, gingivitis, a me ka pepehi ʻana i nā ʻōpū maʻi

LPS manaʻoʻiʻo ia he mea pono i ka mālama ʻana o ka gingivitis a me paradentosis. Ua hoʻohana ʻia ʻo LPO i loko o kahi waha e holoi i nā maʻi huelo e hoʻohaʻahaʻa ʻia, a ma muli o kēia, hoʻohuihui ʻia e kēia mau mea maʻi. Ua hōʻike ʻia nā konupora antibody o ke ʻano lactoperoxidase a me ka glucose oxidase i ka hopena i ka luku ʻana a make i nā aʻa huakini i vitro. Eia kekahi, hoʻohana ʻia ka macrophages i ka ʻōnaehana lactoperoxidase e luku a make i nā maʻi maʻi maʻi maʻi.

iii. Ka mālama waha

Ua hōʻike ʻia nā haʻawina like ʻole o ka maʻi maʻi e wehewehe ana i ka pono o ka LPS i loko o ka niho niho. Ma hope o ke hōʻike like ʻole, me ka hoʻohana ʻana i ke ana ʻana i nā hualoa o nā ʻano caries hopena, ʻo kēlā niho lactoperoxidase e loaʻa ana i ka amyloglucosidase (γ-amylase) i nā hopena maikaʻi i ka mālama waha. ʻO nā huaʻawa e like me ka glucose oxidase, lysozyme, a me ka lactoperoxidase e hoʻoneʻe pololei mai ka palaka ʻana a hiki i ka pellicle.

ʻO nā mea noho i ka pellicle, ʻo kēia mau enzyme e hoʻoikaika nui i ka catalytically. Eia kekahi, loaʻa i ka LPS kahi hopena maikaʻi o ka pale ʻana i nā kahu liʻiliʻi o ka wā kamaliʻi ma o ka hoʻohaʻahaʻa ʻana i ka nui o nā koloni i hoʻokumu ʻia e ka cariogenic microflora i mea e hoʻokiʻekiʻe ai i ka ʻike o thiocyanate.

Me nā maʻi maʻi xerostomia, wili lole lactoperoxidase ʻoi aku ka maikaʻi ma mua o ka hoʻohālikelike ʻana o ka niho fluoride i ka wā e hele mai ai i ka hoʻopiʻi pipi. ʻAʻole paʻa ʻia ke noi o LPS i ka periodontitis a me nā uku māmā. Hiki ke hoʻohui pū me kahi lactoperoxidase a me ka lysozyme i ka mālama ʻana i ka maʻi lapaʻau ʻoi.

Ke hui pū ʻo LPS me ka lactoferrin, ua hui pū kēia hui me ka halitosis. Ke hui pū ʻo LPS me ka lysozyme a me ka lactoferrin, kōkua nā LPS i ka hoʻomaikaʻi ʻana i nā hōʻailona xerostomia. Eia kekahi, nā mea kanu me ka lactoperoxidase kōkua e hoʻomaikaʻi i nā hōʻailona maʻi i ka wā o ka hōʻemi ʻana i ka lawena wai ma muli o ka irradiation.

Lactoperoxidas-04

iv. Hoʻopili i ka ʻōnaehana pale

ʻO ka hana antimicrobial Lactoperoxidase hana i ka hana koʻikoʻi ma ka pale i ka pale ʻana. ʻO Hypothiocyanite kahi hana react i hana ʻia e ka hana lactoperoxidase ma thiocyanate. ʻO ka hydrogen peroxide e hoʻohua ʻia e nā protein Duox2 (ʻelua oxidase 2). ʻO ka ʻōlelo huna Thiocyanate i nā maʻi me ka cystic fibrosis e hoʻemi ʻia. Hoʻopili kēia ma ka hōʻemi o ka hana o ka hypothiocyanite antimicrobial. Hāʻawi kēia i nā kūlana kiʻekiʻe o ka maʻi airway.

Hoʻopā maikaʻi ka LPS i ka helicobacter pylori maikaʻi. Akā i loko o nā wai āpau a ke kanaka, hōʻike ʻo LPS i ka nāwaliwali i nā hopena anti-bacteria. ʻAʻole kū ka LPS i ka DNA a ʻaʻole ia mutagenic. Akā, ma lalo o nā kūlana kūikawā, hiki i ka LPS ke hōʻeha iki i ka hoʻoweliweli oxidative. Ua hōʻike ʻia ʻo LPO i loko o ka thiocyanate hiki ke hoʻowalewale i ka hopena cytotoxic a me ka bactericidal o ka hydrogen peroxide ma lalo o nā kūlana kūikawā, e komo pū ana me ka H2O2 i ka hui ʻana o ka hopena ma ka ʻoi aku o ka thiocyanate.

Eia kekahi, ma muli o kona mau ikaika a me ka maikaʻi o ka maʻi antibacterial a me ka mālama ʻana i ka wela wela, ʻo ia ke ʻano he mea hōʻawaʻawa anti antibacterial no ka hoʻēmi ʻana i nā kaiāulu i ka waiū a i nā huahana waiū a ma ke ʻano o ka waiū ultra-pasteurization. Ma ka hoʻonoho ʻana i ka ʻōnaehana lactoperoxidase, hiki ke hoʻonui ʻia ke ola o ka noho o ka wai me ka wai hoʻoluʻu.

A, ʻo hypothiocyanate i hana ʻia e lactoperoxidase hiki ke hoʻohana ʻia e pale i ka virus herpes simplex a me nā maʻi immunodeficiency kanaka.

Lactoperoxidas-05

Noho palekana paha no ke olakino a me nā holoholona?

He ʻelima mau makahiki o nā noi kula i ke kūkulu ʻana a me nā ʻāina hoʻomohala i hana ʻia a nānā ʻia e ka FAO / WHO JECFA (Komite Hui Hoʻohui e pili ana i nā meaʻai hoʻohui). Ma hope o kēia mau haʻawina hohonu a me ka nui, ua ʻae ʻia ka hoʻohana ʻana o ka ʻōnaehana lactoperoxidase i ka mālama mālama waiu e ka FAO / WHO JECFA (Komite Hoʻohui no ka Hoʻohui Mea ʻai). Ua hōʻike pū ka poʻe loea i kēia ʻano e like me ka palekana no ka pono kino o ke kanaka a me nā holoholona

ʻO ka LPS kahi mea kūlohelohe wai o ka wai momona a me ka lawena i loko o nā kānaka a no laila, palekana i ka wā e hoʻohana ʻia ai i nā ʻōlelo kuhikuhi a Codex Alimentarius Commission. ʻAʻole pili kēia ʻano i nā holoholona lactating i kēlā me kēia. ʻO kēia no ka mea, e hana ʻia ka hoʻomaʻemaʻe wale ʻana ma hope o ka kiʻi o ka waiū mai ka kaat.

Panina

ʻIke ʻia ia mai kā mākou kūkākūkā he maikaʻi loa ka lactoperoxidase a me ka ʻōnaehana lactoperoxidase i ka nui o nā noi. Inā ʻoe e nānā e hoʻomaʻamaʻa ke kūʻai lactoperoxidase no kāu noiʻi 'ole a i ʻole hoʻohana i ka lāʻau lapaʻau, mai nānā hou aku. Hiki iā mākou ke hoʻomaʻamaʻa i nā kauoha bulk lactoperoxidase i ka manawa pōkole loa a hoʻouna iā lākou i ka US, Europe, Canada, a me nā ʻāpana ʻē aʻe o ka honua. E ʻoluʻolu e hoʻokaʻaʻike mai iā mākou no ka ʻike hou aku.

E hoʻomaopopo '

  1. Jantschko, PG Furtmüller, M. Allegra et al., "Redox intermediates o ka lāʻau a me nā mammalian peroxidases: kahi hoʻohālikelike hoʻomāliʻiwahine-kinetic o kā lākou ʻano e pili ana i ka hana indivatives," Archives of Biochemistry and Biophysics, vol. 398, ʻaʻole. 1, pp. 12–22, 2002.
  2. Tenovuo JO (1985). "ʻO ka ʻōnaehana peroxidase i nā ʻike kanaka." Ma Tenovuo JO, Pruitt KM (eds.). ʻO ka ʻōnaehana Lactoperoxidase: ka ʻenekema a me nā ʻike olakino. Nu Ioka: Dekker. p. 272.
  3. ʻO Thomas EL, Bozeman PM, E aʻo iā DB: Lactoperoxidase: ka hoʻonohonohoʻana a me nā waiwai catalytic. Peroxidases i ka Chemistry a me Biology. I hoʻoponopono ʻia e: Everse J, Everse KE, Grisham MB. 1991, Boca Raton, FL. CRC Press, 123-142.
  4. Wijkstrom-Frei C, El-Chemaly S, Ali-Rachedi R, Gerson C, Cobas MA, Forteza R, Salathe M, Conner GE (ʻAukake 2003). "Lactoperoxidase a me ke kahua o ka hoʻāla kaua ea kanaka." Am. J. Respir. ʻAno Mol. Biol. 29 (2): 206–12.
  5. Mikola H, Waris M, Tenovuo J: Inhibition of herpes simplex virus type 1, respiratory syncytial virus, and echovirus type 11 by peroxidase-generated hypothiocyanite. Antiviral Res. 1995, 26 (2): 161-171.
  6. Haukioja A, Ihalin R, Loimaranta V, Lenander M, Tenovuo J (September 2004). "Ka hoʻoweliweli ʻana o ka Helicobacter pylori i kahi mākaʻikaʻi pale kino, ka ʻōnaehana lactoperoxidase, ma ka pōʻai wai a me ka hilo āpau kanaka". Journal of Medical Microbiology. 53 (Pt 9): 855–60.

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